Long-time low-temperature transportation of human ovarian tissue before cryopreservation

Long-Time Cooling before Cryopreservation Decreased Translocation of Phosphatidylserine (Ptd-L-Ser) in Human Ovarian Tissue

OBJECTIVES To translocation (externalization) of phosphatidylserine lead at least the five negative effects observed during cells cryopreservation: hypoxia, increasing of intracellular Ca2+, osmotic disruption of cellular membranes, generation of reactive oxygen species (ROS) and lipid peroxidation. The aim of this study was to test the intensiveness of the phosphatidylserine translocation imme...

Human Ovarian Tissue Cryopreservation as Fertility Reserve

Ovarian tissue and oocyte cryopreservation

There are relatively few effective clinical options for preserving female fertility, particularly following aggressive chemotherapy and/or radiotherapy treatment protocols. This document reviews the scientific background, current technology, clinical results and potential future applications of two methods for preserving female fertility—ovarian tissue cryopreservation and oocyte cryopreservati...

I-10: Vitrification of Baboon Ovarian Tissue:Will Vitrification Replace Conventional Freezing for Ovarian Tissue Cryopreservation?

Background: The aim of this study was to evaluate the efficacy of a vitrification protocol developed by our group to cryopreserve ovarian tissue. For this, we used ovaries from non-human primates in order to have an animal model close to the clinical setting. Materials and Methods: Ovarian biopsies from five adult baboons were vitrified, warmed and autografted. After five months, follicle survi...

Cryopreservation of Ovarian Tissue in Pediatric Patients

Cancer treatments improve the survival rate of children and adolescents; however chemo- and radiotherapy result in gonadal damage leading to acute ovarian failure and sterility. Ovarian tissue cryopreservation allows long-term storage of primordial follicles and represents the only possibility of preserving the potential fertility in prepubertal girls. The aim of the present study is to describ...